background:

β-N- Acetylhexosaminidase is an important enzyme for glycosylation research. Rhinogen ^® β-N- Acetylhexosaminidase is a recombinant enzyme expressed in Escherichia coli BL21 by recombinantly expressing the β-N- Acetylhexosaminidase gene. It can catalyze the release of β(1-3,4,6)-linked N-acetylglucosamine ( GlcNAc ) and β(1-4)-linked N-acetylgalactosamine (GalNAc) residues at the non-reducing end of oligosaccharides.

 Packing Specifications:

Rhinogen ^® β-N- Acetylhexosaminidase packaging specifications are as follows:

Storage system:

QPF-007 is provided in liquid form in a buffer consisting of 150 mM NaCl, 20 mM Tris-HCl (pH 7.5, 25°C), and 5 mM EDTA.

Supporting reagents:

Rhinogene ® The supporting reagents provided by β-N -Acetylhexosaminidase are as follows:

Product Source: 

Rhinogen ^® β-N -Acetylhexosaminidase is a recombinant enzyme that is produced by recombining the β-N- Acetylhexosaminidase gene and expressing it in E. coli BL21. Its molecular weight is 55KD.

Product Quality:

SDS-PAGE analysis showed that the purity was ≥95%; no contaminating exoglycosidase, endoglycosidase and protease activities were detected.

Product Features:

The optimal reaction pH is 5.5. Inactivation conditions: 75°C for 10 min.

Storage conditions:

-20°C.

Features:

Acetylhexosaminidase product provided by ^Rhinogen® has the characteristics of high stability and high specific activity. It is a highly purified and very stable exoglycosidase, suitable for the non-reducing terminal β(1-3,4,6)-linked N-acetylglucosamine ( GlcNAc ) and β(1-4)-linked N-acetylgalactosamine (GalNAc) residues of glycoproteins in proteomics and glycobiology research.

1. High purity: no contaminating protease/other glycosidase, purity ≥95%;

2. High stability: Each batch of β-N -Acetylhexosaminidase products undergoes strict quality control and exhibits high stability;

3. High specific activity: effectively releases N-acetylglucosamine and N-acetylgalactosamine residues at the non-reducing end.

application:

1. Glycan structure analysis;

2. Characterization and quality control of therapeutic recombinant proteins;

3. Eliminate glycoprotein heterogeneity.

Recommended use:

1. Take 1µg of glycoprotein or 100nM oligosaccharide sample and add purified water to the reaction system to 8μl;

2. Add 1µl 10× Glyco Buffer 1;

3. Add 1 µl Rhinogen ^® β-N- Acetylhexosaminidase and mix gently;

4. Incubate at 37°C for 1 hour.

Instructions:

1. For different glycoprotein samples, it is necessary to experiment to find the most suitable enzyme concentration and reaction time;

2. The reaction can be scaled up or down linearly;

3. For different glycoprotein samples, the required enzyme amount needs to be properly optimized or the optimal operation method needs to be determined based on experience. In a 10-25μl reaction system, for 1μg of glycoprotein, the recommended initial enzyme amount is 1-2μl and the reaction is for 1 hour. If it is not completely digested, it is recommended to treat overnight;

4. This product is for research use only and is not intended for diagnosis or treatment of humans or animals.