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In primary culture, it is often necessary to digest and dissociate tissue blocks to form a cell suspension, and in subculture, it is also necessary to digest adherent cells from the flask wall. Trypsin is a serine protease that can hydrolyze proteins between cells and destroy the connection between cells, thereby dispersing tissues or adherent cells into single cells.

Packing Specifications:

Rhinogen ^® recombinant trypsin cell digestion solution packaging specifications are as follows:

Note: This product has been sterile filtered, pH is 7.2~8.0, and osmotic pressure is 270-320 mOsm /kg.

Product activity:

This product contains recombinant trypsin with a specific activity (unit/mg) ≥ 4500U/mg.

Storage conditions:

Use ice packs for transportation. After receiving the product, please store it at -20℃ for long-term storage. Try to reduce repeated freezing and thawing (6 freeze-thaw experiments can maintain good digestion effect). If multiple freeze-thaw experiments are required, it is recommended to package it; the digestion effect is good when stored at 2-8℃ for 1 month.

Product Features:

1. High purity: recombinant production, chromatographic purification, free of impurities such as enzymes, proteins, lipids, and DNA;

2. Strong digestion ability: room temperature digestion, high purity, avoiding the influence of impurities on cell growth;

3. No animal origin: Recombinant production ensures no animal origin virus contamination.

application:

1. Used for digestion of host cells during vaccine production;

2. Digestion during the passage of stem cells and primary cells;

3. Digestion of adherent cells during the production of recombinant proteins.

Precautions for use:

1. When using, take out at -20℃ and dissolve at room temperature before use. Do not preheat directly at 37℃.

2. The digestion time required for different types of cells is slightly different, so it needs to be explored according to the actual situation;

3. When using this product, special attention should be paid to avoid bacterial contamination of digestive fluid;

4. This product is limited to scientific research by professionals and shall not be used for clinical diagnosis or treatment, food or medicine, or stored in ordinary residences;

5. For your safety and health, please wear a lab coat and disposable gloves when operating.