background:

DMEM/F-12 medium ( Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 ) is a basic medium widely used in the culture of a variety of mammalian cells, including human epidermal cells, fibroblasts, MDCK , glial cells, human endothelial cells, rat fibroblasts, etc. DMEM/F-12 is an equal proportion of DMEM and Han 's F-12 medium, combining the advantages of the two mediums. This medium does not contain proteins, lipids and growth factors, so when used for cell culture, it is usually necessary to add 10% fetal bovine serum.

Specifications:

Storage conditions:

2-8 ℃, protect from light;