background:

Zeocin is a glycopeptide copper ion chelating antibiotic isolated from Streptomyces verticillus . Zeocin is light blue. When chelated with copper ions, zeocin has no toxic effect. When it enters the cell , the copper ions are reduced and removed. At this time, zeocin will exert its toxicity by inserting into DNA , causing DNA to break and leading to cell death. ble encodes a protein of about 14 kDa that can make cells expressing this gene resistant to Zeocin . This protein can bind to the Zeocin antibiotic and prevent it from binding to DNA , thus making cells resistant to Zeocin .

In Escherichia coli , the recommended selection concentration of bleomycin is 25-50 μg /ml , and LB plates containing bleomycin ( yeast extract 5 g/l, Tryptone 10 g/l, NaCl 5 g/l, Agar 15 g/l, pH 7.5 ) can be stored at 4 °C for 1 month. In mammalian cells, the selection concentration of bleomycin ranges from 50-1000 μg /ml , usually 250-400μg/ml , which should be optimized for each cell line.

use:

Used for selection of drug-resistant cells in bacterial, fungal transformation and mammalian transfection systems.

Packing Specifications:

Storage conditions/stability:

Transport frozen. Store at -20 ℃ away from light. If stored properly, it can be kept stable for 6 months.

Note:

1. Please wear gloves, lab coat and protective glasses when handling bleomycin solution;

2. Bleomycin is sensitive to light. Please keep the resistant plates and culture media containing bleomycin away from light;

3. When using bleomycin as a screening antibiotic for E. coli , please use low-salt ( NaCl 5 g/L ) LB medium and adjust the pH to 7.5 . Overly acidic or alkaline medium will inhibit the activity of bleomycin.

4. Please store bleomycin at -20 ℃ and melt on ice before use.