background:

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus . It inhibits protein synthesis by mistranslating mRNA templates, thereby killing bacteria, fungi, and eukaryotic cells. The gene hyg or hph can provide hygromycin B resistance to cells expressing the gene.

In Escherichia coli, the recommended selection concentration range of hygromycin B is 50-100 μg/ml. LB plates containing hygromycin B (yeast extract 5 g/l, Tryptone 10 g/l, NaCl 5 g/l, Agar 15 g/l, pH 7.5) can be stored at 4°C for 1 month. In mammalian cells, the recommended selection concentration range of hygromycin B is 100-800 μg/ml, which should be optimized for each cell line. The onset time of hygromycin B is longer than that of G418. Depending on the cell type, it usually takes 10 days to 3 weeks of culture to obtain a stable cell line.

Chemical Information:

CAS No.: 31282-04-9

Chemical formula: C ₂₀ H ₃₇ N ₃ O ₁₃

Molecular weight: 527.54

use:

Used for selection of drug-resistant cells in bacterial, fungal transformation and mammalian transfection systems.

Packing Specifications:

Storage conditions/stability:

Transport at room temperature; store at 2-8℃ away from light. Store at 37℃ for 1 month; store at 2-8℃ for 2 years; store at -20℃ for 2 years.

References:

1. Merck Index, 12th ed., 4900 (1996).