background:

G418 is a kind of An aminoglycoside antibiotic produced by rhodorangea , its structure is similar to gentamicin . It inhibits protein synthesis in prokaryotic and eukaryotic cells by binding to ribosomes, leading to cell death. The neomycin resistance gene ( neo ) encodes APH3'II , which can make cells expressing this gene resistant to G418 .

In Escherichia coli , the recommended selection concentration range of G418 is 100-200 μg /ml . In mammalian cells, the selection concentration range of G418 is 400-800 μg/ml and should be optimized for each cell line.

Chemical Information:

CAS No.: 108321-42-2

Chemical formula: C ₂₀ H ₄₀ N ₄ O ₁₀ • 2H ₂ SO ₄

Molecular weight: 692.7

use:

Used for selection of drug-resistant cells in bacterial, fungal transformation and mammalian transfection systems.

Packing Specifications:

Storage conditions/stability:

Transport at room temperature; store at 2-8 ℃ away from light. It can be stored for 2 years at 2-8 ℃ away from light . It can be stored stably for 8 days at 37 ℃.