background:

Rhinogen ^® recombinant anti-human IgG (Fc) nanobody - Fluorescein tag is obtained by recombinant expression, which can specifically recognize 4 types of human IgG and has ultra-high affinity. The Fluorescein tag can be excited by 480nm laser and emit at 510nm. It can be used for flow cytometry analysis (Flow), immunofluorescence (IF), immunocytochemistry (ICC), immunohistochemistry (IHC), etc. In particular, it can be used in conjunction with flow cytometry sorters, ClonePix ™ Systems, and F.sight ™ single cell screening instruments for high-expression IgG clone screening, and a large number of high-expression clones can be obtained in a short time .

Host Cells: 

Chinese Hamster Ovary (CHO) cells, cultured in animal origin-free medium.

Specifications:

composition:

Liquid (PBS, pH 7.2).

characteristic:

1. No animal-derived ingredients are exposed during the entire production process, and there is no risk of exogenous factors contaminating the screening cells, with high regulatory compliance;

2. High specificity and high affinity binding to 4 different types of human IgG;

3. Antibodies do not need to be labeled and the secretion expression of IgG can be directly detected;

4. Sterility;

5. No sodium azide;

6. Low endotoxin;

7. Specific recognition of Fc fragment;

8. High fluorescence value: After incubation with the same antibody concentration, the fluorescence value of flow cytometric analysis was higher than that of Goat anti- HumanIgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 ( Thermo Company, cat : A11013).