Antigen Name:

Recombinant human FcγRIIa 131H (His tag).

background:

The Fc region receptor of IgG (FcγR) is a member of the Ig superfamily and has the function of activating or inhibiting immune responses. There are three types of human FcγRs, RI (CD64), RII (CD32), and RIII (CD16), which can produce multiple isoforms. Human FcγRII/CD32 has three genes (A, B, and C), and mouse FcγRII B has one gene (CD32B). CD32 is a low-affinity IgG receptor. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets, and dendritic cells. CD32A is expressed on many immune cell types (macrophages, neutrophils, eosinophils, platelets, dendritic cells, and Langerhans cells), among which receptors containing inhibitory ITIMs may also be co-expressed and co-engaged by specific ligands. CD32A sends an activation signal upon ligand binding, leading to the initiation of inflammatory responses, including cell lysis, phagocytosis, degranulation, and cytokine production. These responses can be modulated by signals from co-expressed inhibitory receptors such as CD32B, and the strength of the signal depends on the expression ratio of activating and inhibitory receptors.

Sequence source:

Recombinant human FcγRIIa 131H (Ala 36 - Ile 218) is expressed in Chinese hamster ovary (CHO) cells and is His-tagged.

Molecular Weight:

The molecular weight of SDS-PAGE reduction electrophoresis was 29-32 kDa.

Specifications:

purity:

The purity in SDS-PAGE reduction electrophoresis was >95%.

Biological activity:

Using BLI detection ( ForteBio Octet Red96e), recombinant human FcγRIIa 131H (His tag) was loaded on the HIS1K biosensor and its affinity constant for binding to trastuzumab was determined to be 2.75 μM.

concentration: 

1 mg/ml.

Buffer:

20mM PB, 0.15M NaCl, pH7.4.

Storage conditions:

For long-term storage, this product should be stored at -20°C or lower. Please avoid repeated freezing and thawing.