Antigen Name:

Trastuzumab specific antigen (His tag), a dedicated antigen for antibodies that specifically bind to the IV region of the extracellular region of ErbB2 (NP_004439.2).

background:

Human epidermal growth factor receptor 2 (Her-2) is a transmembrane glycoprotein with receptor tyrosine kinase (RTK) activity encoded by the proto-oncogene CerbB2 (Her2/neu). It belongs to the epidermal growth factor receptor family, which includes HER1 (erbB1, EGFR), HER2 (erbB2, NEU), HER3 (erbB3) and HER4 (erbB4). Studies have shown that ErbB2/HER2 has gene amplification and protein overexpression in 20% to 30% of primary invasive ductal breast carcinoma. Trastuzumab and Pertuzumab are both monoclonal antibodies that bind to the extracellular domain of ErbB2/HER2, but the two have different binding sites. Pertuzumab binds to the extracellular domain II of the ErbB2/HER2 receptor, while trastuzumab binds to the extracellular domain IV of the HER2 receptor.

Sequence source:

The trastuzumab specific antigen (His tag) is a mutant of the extracellular domain II of full-length human ErbB2 (NP_004439.2) expressed in Chinese hamster ovary (CHO) cells.

Molecular Weight:

The molecular weight in SDS-PAGE reduction electrophoresis is 100-110 kDa.

Reagent packaging:

Buffer: 10% glycerol, 100 mM Gly, 25 mM Tris, pH 7.3.

application:

Recombinant human ErbB2 ECD-IV region specific antigen can be used to quantitatively detect the concentration of monoclonal antibodies that specifically bind to ErbB2 IV region, such as Trastuzumab, in cell culture medium, animal and human serum, and dairy products.

purity:

Purity in SDS-PAGE reduction electrophoresis>95%;

concentration:

1mg/ml.

Storage conditions:

This product can be stored at -70℃ for 36 months; at -20℃ for 12 months; at 2~8℃ for 1 month.

Please avoid repeated freezing and thawing.