background:

The human NPP family contains seven members that can be divided into two groups based on their substrate preferences. The first group includes the nucleotide-degrading proteins NPP1, 3, and 4. NPP3 (CD203c, ENPP3) is expressed in multiple organs, including epithelial and mucosal surfaces, especially basophils and mast cells. Activation of basophils by antigen-bound IgE leads to the release of inflammatory mediators and a rapid upregulation of NPP3 to the cell surface. In fact, this protein is a common marker for the diagnosis of basophil allergy in patients by flow cytometry. Basophils and mast cells mediate responses to certain pathogens, as well as acute and chronic allergic reactions. Upon activation, these cells release ATP, which further stimulates them in an autocrine manner. NPP3 upregulation helps degrade ATP and suppress chronic allergic inflammation (but not acute reactions).

Overview:

Antigen name : Human ENPP3 / CD203c Protein, His Tag;

Other names : ENPP3, CD203c, NPP3, E-NPP3, PD- Ibeta , NPPase , PDNP3;

Sequence source : human ENPP3, His tag (EN3-H52H4) expressed from human 293 cells (HEK293) with a His tag;

Molecular Weight: The calculated molecular weight of this protein is 96.9 kDa . Due to glycosylation, the protein migrates at 115-120 kDa and 121-130 kDa under reducing conditions (SDS-PAGE) .

Packing Specifications:

Rhinogen® recombinant human ENPP3 / CD203c protein (His tag) packaging specifications are as follows ^:

characteristic:

Purity: Purity in SDS-PAGE reduction electrophoresis> 90%.

Storage temperature:

This product can be stored in a freeze-dried state at -20°C to -70°C for 12 months; after reconstitution, it can be stored at -70°C under sterile conditions for 3 months. Please avoid repeated freezing and thawing.