background:

dickkopf -related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. DKK1 participates in embryonic development by inhibiting the WNT signaling pathway, binds to LRP6 with high affinity, and prevents the formation of the Frizzled-Wnt-LRP6 complex in response to Wnts . DKK1 promotes the internalization and degradation of LRP6 when it forms a ternary complex with the cell surface receptor Kremen. DKK1 does not act as a head inducer during development, but also regulates joint remodeling and bone formation , suggesting a role for DKK1 in the pathogenesis of rheumatoid arthritis and multiple myeloma. Recent studies report that DKK1 affects eye development from a defined developmental time point and is essential for the separation of the lens from the surface ectoderm through β-catenin -mediated Pdgfr α and E-cadherin expression.

Overview:

Antigen name : Human Dkk-1 Protein, Fc Tag;

Other names : DKK1, SK type;

Sequence source : human Dkk-1, Fc-tagged DK1-H5258) expressed by human 293 cells (HEK293), with His tag;

Molecular Weight : The calculated molecular weight of this protein is 52.4 kDa . Due to glycosylation, the protein migrates at 67-85 kDa under reducing conditions (SDS-PAGE) .

Packaging specifications:
Rhinogen ^® recombinant human Dkk-1 protein (Fc tag) packaging specifications are as follows:

Storage temperature:

This product can be stored in a freeze-dried state at -20°C to -70°C for 12 months; after reconstitution, it can be stored at -70°C under sterile conditions for 3 months. Please avoid repeated freezing and thawing.

Characteristics:
Purity: Purity in SDS-PAGE reduction electrophoresis>95%.