background:

Myeloid cell surface antigen CD33, also known as SIGLEC3, Siglecs (sialic acid-binding Ig-like lectin) and GP67, is a single-pass type I membrane protein belonging to the immunoglobulin superfamily and the SIGLEC (sialic acid-binding Ig-like lectin) family. Human CD33/Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, an Ig-like C2-type domain, a transmembrane region and a cytoplasmic tail. CD33/Siglec-3 is generally considered to be myeloid-specific, but it can also be found on some lymphocytes. In immune responses, CD33/Siglec-3 may recruit cytoplasmic phosphatases through its SH2 domain, blocking signal transduction by dephosphorylation of signaling molecules, thereby acting as an inhibitory receptor upon ligand-induced tyrosine phosphorylation. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia cells.

Overview:

Antigen name: Human Siglec-3 / CD33 Protein, Fc Tag;

Other names: CD33, SIGLEC3, GP67;

Sequence source: Human Siglec-3, Fc tag (CD3-H5257) expressed by human 293 cells (HEK293) with Fc tag;

Molecular Weight: The calculated molecular weight of this protein is 52.9 kDa . Due to glycosylation, the protein migrates at 64-85 kDa under reducing conditions (SDS-PAGE) .

Packaging specifications:
Rhinogen® recombinant human Siglec-3 / CD33 protein (Fc tag) packaging specifications are as follows ^:

Storage temperature:

This product can be stored in a freeze-dried state at -20°C to -70°C for 12 months; after reconstitution, it can be stored at -70°C under sterile conditions for 3 months. Please avoid repeated freezing and thawing.

Characteristics:
Purity: Purity in SDS-PAGE reduction electrophoresis>95%.