Antigen Name:

Recombinant human Siglec-3 / CD33 protein (His Tag)/Human Siglec-3 / CD33 Protein, His Tag;

Other Names:

CD33, SIGLEC3, gp67;

background:

Myeloid cell surface antigen CD33, also known as SIGLEC3, Siglecs (sialic acid binding Ig-like lectins) and GP67, is a single-channel type I membrane protein belonging to the immunoglobulin superfamily and the SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33/Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, an Ig-like C2-type domain, a transmembrane region and a cytoplasmic tail. CD33/Siglec-3 is generally considered to be myeloid-specific , but can also be found on certain lymphocytes. In immune responses, CD33/Siglec-3 can act as an inhibitory receptor upon ligand-induced tyrosine phosphorylation, recruiting cytoplasmic phosphatases through its SH2 domain, thereby blocking signal transduction by dephosphorylation of signaling molecules. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia.

Sequence source:

Human Siglec-3 / CD33 Protein, His Tag is expressed by Chinese Hamster Ovary (CHO) cells; the protein number is AAH28152.1 (Asp18 - His259), with His tag;

Molecular Weight:

The theoretical molecular weight is 28.64 kDa. Due to glycosylation, the molecular weight of SDS-PAGE reduction electrophoresis is 43-55 kDa.

Specifications:

purity:

SDS-PAGE electrophoresis purity> 99%;

application:

Monoclonal antibody used for quantitative detection of HumanSiglec-3 / CD33 Protein in cell culture medium, serum and milk.

Buffer:

20mM PB, 150mM NaCl, pH7.4;

Storage conditions:

This product can be stored at -20℃~-70℃ for 12 months; after reconstitution, it can be stored at -70℃ under sterile conditions for 3 months. Please avoid repeated freezing and thawing.