background:

Chondrocytes have certain cell activity and can participate in the repair and regeneration of cartilage tissue to respond to external stimuli and damage. In medical research, chondrocytes can be used to study and treat diseases related to cartilage damage and loss, such as osteoarthritis, cartilage defects, and trauma. In addition, chondrocytes can also be used to make artificial organs, such as artificial ears, noses, joints, etc. They can also be used to screen drugs and study pharmacology, as well as to study the effects and mechanisms of autoimmune diseases on cartilage tissue.
The chemical name of collagenase is collagenase, which can cleave the bond between the neutral amino acid X and glycine, and its sequence is Gly-XY-Gly-XY, which is found frequently in collagen. It can specifically hydrolyze the three-dimensional helical structure of natural collagen under physiological pH and temperature conditions, such as skin, tendons, blood vessels and bones, without damaging other proteins and tissues. Collagenase decomposition is applicable to the culture of human tumors, mouse kidneys, human adult and fetal brains and many other tissues including epithelial cells. The chemical nature of collagenase is a protein. Therefore, it is very sensitive to temperature, pH and various factors that cause protein denaturation. It is easily affected by external conditions and changes its own conformation and properties. Thermolysin is a heat-stable metalloprotease. High digestion temperature acts as a selective denaturant to promote the digestion of proteins that are resistant to proteolysis. Thermolysin preferentially cleaves at the N-terminus of hydrophobic amino acid residues, including leucine, phenylalanine, valine, isoleucine, alanine and methionine. Its optimal digestion temperature range is between 65-85°C. The optimal pH range for thermolysin enzyme activity is 5.0-8.5.

Overview:

Rhinogen ^® recombinant collagenase (recombinant collagenase G and recombinant collagenase H) is derived from Clostridium histolyticum and is produced by recombinant expression in the E. coli system. It can be used for the dissociation of different functional cells and has the characteristics of high purity, good stability, small batch-to-batch variability and no animal origin. The two collagens, recombinant collagenase G and recombinant collagenase H, can be mixed into a specific formula for each application.
Recombinant collagenase H and thermolysin can be mixed for the separation of chondrocytes.

application:

Applied to the isolation of chondrocytes.

Reagent packaging:

Product Features:

1. High purity: >95%, no contaminating protease, can reduce over-digestion;
2. High adaptability: adjust the collagenase digestion rate according to the experimental application;
3. No animal origin: recombinant production, no exogenous virus contamination, and no animal origin raw materials are used in the production process;
4. High stability: each batch of products undergoes strict quality control to achieve product batch-to-batch stability ;
5. Low endotoxin: no side effects on cells.

Storage conditions:

Transport with ice packs, store at -20℃, and seal to prevent moisture. After reconstitution, please divide into portions according to the single use amount and store at -20℃ to avoid repeated freezing and thawing.