background:

Rat liver cells can be used for liver physiology and pathology research, as well as drug toxicity assessment. By isolating and culturing rat liver cells, the physiological and pathological states of the liver can be simulated in vitro, liver function and the pathogenesis of diseases can be assessed, and they are also widely used in drug screening and toxicity assessment. For example, liver cell metabolism, toxin metabolism and the pathogenesis of liver diseases can be studied, and they can also be used to screen drugs such as liver protectants and antidotes. In addition, rat liver cells can also be used for medical research in the fields of liver cancer and liver transplantation.

The chemical name of collagenase is collagenase, which can cleave the bond between the neutral amino acid X and glycine. Its sequence is Gly-XY-Gly-XY, which is found frequently in collagen. It can specifically hydrolyze the three-dimensional helical structure of natural collagen under physiological pH and temperature conditions, such as skin, tendons, blood vessels and bones, without damaging other proteins and tissues. Collagenase decomposition is applicable to human tumors, mouse kidneys, human adult and fetal brains and many other tissues including epithelial cell culture. The chemical nature of collagenase is a protein, so it is very sensitive to temperature, pH and various factors that cause protein denaturation, and is easily affected by external conditions to change its own conformation and properties.

Thermolysin is a thermostable metalloprotease. High digestion temperature acts as a selective denaturant to promote the digestion of proteins that are resistant to proteolysis . Thermolysin preferentially cleaves at the N - terminus of hydrophobic amino acid residues, including leucine, phenylalanine, valine, isoleucine, alanine and methionine. Its optimal digestion temperature range is between 65-85°C. The optimal pH range for thermolysin enzyme activity is 5.0-8.5.

Overview:

Rhinogen ^® recombinant collagenase (recombinant collagenase G and recombinant collagenase H) is derived from Clostridium histolyticum and is produced by recombinant expression in the E. coli system. It can be used for the dissociation of different functional cells and has the characteristics of high purity, good stability, small batch-to-batch variability and no animal origin. The two collagens, recombinant collagenase G and recombinant collagenase H, can be mixed into specific formulas for each application.

Recombinant collagenase G, recombinant collagenase H and thermolysin can be used in combination to isolate rat liver cells.

application:

Applied to the isolation of rat liver cells.

Reagent packaging:

the Rhinogen ^® Rat Liver Cell Isolation Kit are as follows:

Product Features:

1. High purity: >95%, no contaminating protease, can reduce over-digestion;

2. High adaptability: adjust the collagenase digestibility according to the experimental application;

3. No animal origin: Recombinant production, no exogenous virus contamination, and no animal origin raw materials are used in the production process;

4. High stability: Each batch of products undergoes strict quality control to achieve batch-to-batch stability ;

5. Low endotoxin: no side effects on cells.

Storage conditions:

Transport with ice packs, store at -20℃, and seal to prevent moisture. After reconstitution, please divide into portions according to the single use amount and store at -20℃ to avoid repeated freezing and thawing.