background:

Mycoplasma is a general term for prokaryotic microorganisms of the Mycoplasma family, the Acholesteroplasma family , and the Spiroplasma family. It is the smallest known free-living organism, has no cell wall, has a variety of shapes, is generally 0.1-0.3 μm in diameter, has a high AT content in the genome, is insensitive to common antibiotics, and is sensitive to heat. At present, about 200 species of mycoplasmas have been found in sewage, plants, animals, poultry, insects, humans, hot springs, or other high-temperature environments.
If cells are contaminated with mycoplasmas, the cell growth rate slows down, and the cells develop lesions or morphological changes. The probability of continuous culture cell contamination is about 15-35%, mainly from more than 20 species of mycoplasmas, including oral mycoplasma ( M.orale ), pneumonia mycoplasma ( M.pneumoniae ), fermentation mycoplasma ( M.fermentans ), arginine mycoplasma ( M.arginini ), A.laidlawii ( A.laidlawii ), and hyorhinis mycoplasma ( M.hyorhinis ).

Overview:

Rhinogen ^® Myco-EXT™ Mycoplasma DNA Automated Extraction Kit (non-prepacked) uses specially modified silica nanomagnetic microspheres. Under the action of a unique buffer system and an external magnetic field, it can extract trace DNA from complex biological samples and purify high-purity DNA. It can be used in conjunction with the Rhinogen ^® Myco-Acid™ qPCR Mycoplasma Detection Kit (Cat. No.: RA-MT05) to qualitatively detect whether there is mycoplasma contamination in master cell banks, working cell banks, virus seed batches, and cells used for clinical treatment.

Reagent packaging:

the Rhinogen ^® Myco-EXT™ Mycoplasma DNA Automated Extraction Kit (non-prefilled) are as follows:

Storage conditions:

Transported in ice packs. Upon receipt of the product, please immediately store the lysis solution 1 (Cat. No.: RA-MT07A) at 2-8°C and other components at room temperature (15-25°C). The shelf life is 12 months.