background:

Mycoplasma is a general term for prokaryotic microorganisms of the Mycoplasma family, the Acholesteroplasma family , and the Spiroplasma family. It is the smallest known free-living organism, has no cell wall, has a variety of shapes, is generally 0.1-0.3 μm in diameter, has a high AT content in the genome, is insensitive to common antibiotics, and is sensitive to heat. At present, about 200 species of mycoplasmas have been found in sewage, plants, animals, poultry, insects, humans, hot springs, or other high-temperature environments.
If cells are contaminated with mycoplasmas, the cell growth rate slows down, and the cells develop lesions or morphological changes. The probability of continuous culture cell contamination is about 15-35%, mainly from more than 20 species of mycoplasmas, including oral mycoplasma ( M.orale ), pneumonia mycoplasma ( M.pneumoniae ), fermentation mycoplasma ( M.fermentans ), arginine mycoplasma ( M.arginini ), A.laidlawii ( A.laidlawii ), and hyorhinis mycoplasma ( M.hyorhinis ).
Human operations, contaminated cells, raw materials and auxiliary materials (serum, trypsin, culture medium), experimental environment pollution (biosafety cabinets, cell rooms, incubators), experimental instruments (water baths, liquid nitrogen tanks), and experimental consumables (culture dishes, square bottles, cell factories) may all be sources of contamination. On the one hand, contaminated cells have a huge impact on production. On the other hand, if cell products, protein products, and viral products are contaminated with mycoplasma, they will eventually bring potential health risks to patients. Therefore, regulatory authorities require companies to conduct mycoplasma testing on cell banks, test cells, and products, control them from the source, detect them as early as possible, and ensure that released products do not contain mycoplasma. In response to this, drug regulatory authorities in countries around the world have also issued relevant guidelines for mycoplasma testing. The detection methods mainly include fluorescent staining, culture, biochemical detection methods, and nucleic acid amplification methods.

Overview:

Rhinogen ^® Myco- Acid ^TM qPCR Mycoplasma Detection Kit uses the Taqman fluorescent probe principle to qualitatively detect whether there is mycoplasma contamination in master cell banks, working cell banks, virus seed batches, and cells used for clinical treatment.

Reagent packaging:

the Rhinogen ^® Myco- Acid ^TM qPCR Mycoplasma Detection Kit are as follows:

Storage conditions:

Transported by dry ice, please store at -18℃ or below immediately after receiving the product , the validity period is 12 months.
Note: Primer probe mixture (Cat. No.: RA-MT05B) should be stored at -18℃ or below in the dark.