background :

Adenosine triphosphate (ATP) is a common energy carrier in various life activities in nature and is the smallest unit of energy storage and transfer. ATP participates in a variety of enzymatic reactions in organisms, maintains normal life activities, and is an indicator of the metabolism of living cells. ATP is the most important energy molecule in cells. It can be used to measure the level of cell metabolism and has a good linear relationship with the number of living cells. Therefore, the number of living cells can be reflected by the ATP content. By monitoring changes in ATP content, the cell killing, cell inhibition and cell proliferation effects caused by various drugs, biological agents or bioactive substances can be evaluated. The increase in ATP content is also often used as an important indicator of food and drug contamination caused by microbial reproduction.

Cell Titer Turbo 2.0 Luminescent Cell Viability Assay uses the luciferase-catalyzed luciferin luminescence reaction, which is dependent on ATP, to determine the intracellular ATP content through chemiluminescent signals, thereby detecting cell viability or quantitatively detecting the number of live cells. The detection has a wide linear range, high sensitivity, and good stability. In a 96-well plate, there is a good linear relationship in the range of 100 to 100,000 cells, but the upper limit of the detection number of different cells will be different. In addition, the operation is simple, the detection reagent provided in the kit is ready-to-use, the reading is stable, and the detection speed is fast. It only takes about 10 minutes to complete the test . There is no need to wash the cells, replace or remove the culture medium. Compared with other common cell viability determination methods, such as Calcein -AT, CCK-8, etc., Cell Titer Turbo 2.0 Luminescent Cell Viability Assay is simpler and faster.

Specifications:

application:

Cell Titer Turbo 2.0Luminescent Cell Viability Assay is suitable for activity detection of bioactive factors, large-scale anti-tumor drug screening, cell proliferation, cytotoxicity tests, etc.

Operation Introduction:

Product Features:

The CellTiter Turbo 2.0 Luminescent Cell Viability Assay has high detection sensitivity and a wide linear range, and is compatible with the detection of a small number of samples and high-throughput screening of a large number of samples.

1. Convenient and fast: The detection reagents provided in the kit are ready-to-use, with stable readings and fast detection speed. It only takes about 10 minutes to complete the test ;

2. High sensitivity: capable of detecting luciferase as low as 10 ^{-13 mol;}

3. Wide linear range: In a 96-well plate, there is a good linear relationship in the range of 100 to 100,000 cells, but the upper limit of the detection number of different cells may vary;

4. High throughput: compatible with the detection of a small number of samples and high-throughput screening of a large number of samples.

storage:

1. Store below -10℃ away from light. To ensure the best performance, it is recommended to store at -70℃ for long-term storage.

2. This product can remain stable after 6 cycles of freezing and thawing.

3. Avoid subpackaging as it may lead to the risk of ATP contamination.

Frequently asked questions