background :

CCK-8 reagent contains WST-8: Chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonic acid benzene)-2H-tetrazole monosodium salt, which is reduced to a highly water-soluble yellow formazan product (Formazan) by dehydrogenase in the cell mitochondria under the action of the electron carrier 1-methoxy-5- methylphenazine dimethyl sulfate (1-Methoxy PMS). The amount of formazan generated is proportional to the number of living cells. The light absorption value is measured at a wavelength of 450nm using an enzyme-linked immunosorbent assay, which can indirectly reflect the number of living cells. This method has been widely used in the activity detection of some biologically active factors, large-scale anti-tumor drug screening, cell proliferation tests, cytotoxicity tests, and drug sensitivity tests.

Packing Specifications:

advantage:

Compared with the common MTT method, the CCK-8 method has significant characteristics:

1. High sensitivity, reliable data and good reproducibility

2. Easy to operate, saving time and effort

3. Water-soluble, no need to change the solution, especially suitable for suspended cells

4. No need for radioactive isotopes and organic solvents, low cytotoxicity

5. It is a bottle of solution, no need to prepare, it is ready to use

6. Suitable for high-throughput drug screening;

Storage conditions:

CCK-8 can be stored at 0~5℃ for 1 year in the dark, and can be stored longer at -20℃. Repeated freezing and thawing will increase the background value and interfere with experimental determination. If you need to use it frequently, please divide it or store it at 0~5℃.