Overview:

The commonly used method for studying eukaryotic gene expression regulation is to detect reporter genes, and bioluminescence is the most commonly used and effective means of reporter gene detection. Luciferase can catalyze the conversion of substrate luciferin and emit photons. This product provides a fast, sensitive and stable detection method for the expression of firefly luciferase reporter gene in mammalian cells. It can detect as low as ^10-20 mol of luciferase ^and 10-14 The linear relationship was good within the enzyme concentration range of 10-20 mol ^.

Reagent packaging :

Firefly&Renilla Assay Kit ( Dual Luciferase Reporter Gene Assay Kit) packaging specifications are as follows :

Kit Components :

A. 5× Passive Luciferase Lysis Buffer

B. Firefly Luciferase Assay Buffer

C. D-Luciferin

D. Renilla Luciferase Assay Buffer

E. 50× Coelenterazine

Product Features:

1. Rapid : Cell lysis is completed within 10-15 minutes ;

2. Convenient : The reagents are easy to prepare and the sample testing steps are simple;

3. Sensitive : able to detect as little as 10 ^-20 mol of luciferase;

4. Linearity : The linear range of enzyme concentration can reach 8 orders of magnitude.

Product Application:

Studies on the regulation of eukaryotic gene expression.

Storage conditions:

at -20 ℃ in a dry place away from light. Please refer to the outer packaging for the expiration date.