Overview:

Optical in vivo imaging currently mainly uses bioluminescence and fluorescence . The bioluminescence method is based on the principle that luciferase can catalyze the chemiluminescence of substrates. Cell lines that can stably express luciferase in vitro are implanted into animals to react with substrates injected into the body later. The optical system is used to detect the light intensity, which indirectly reflects the changes in cell number or cell location. This technology has been widely used in many fields, the most commonly used being the establishment of tumor or disease animal models, and can be used for virology research, siRNA research, stem cell research, protein interaction research, etc.

D-Luciferin is a common substrate of luciferase and is widely used in the entire biotechnology field, especially in vivo imaging technology. Under the action of ATP and luciferase, luciferin can be oxidized and its chemiluminescence can be detected at 560nm . The luc gene encodes a luciferase that is present as a reporter gene in a variety of cells. Due to the low background of chemiluminescence , the luc gene can be monitored at very low expression levels. In addition, luciferin / luciferase is used to measure 10-15 molar amounts ^of ATP .

Reagent packaging:

D-Luciferin, Potassium Salt ( D- Luciferin Potassium Salt) packaging specifications are as follows:

Product parameters:

Appearance: Light yellow solid soluble in water

Ex/Em ： 328/533 nm

Molecular formula: C ₁₁ H ₇ N ₂ O ₃ S ₂ K

Molecular weight: 318.4

Molecular structure diagram:

Product Application:

1. luc in living cells, tissues or organisms Imaging analysis of marker gene and luciferase - fusion gene expression in vivo / in vitro;

2. Reporter gene assay, immunoassay and ATP fluorescence health monitoring assay.

Storage conditions:

at -20 ℃ in a dry place away from light. Please refer to the outer packaging for the expiration date.