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Firefly luciferase is a protein with a molecular weight of 61kDa. It has no background fluorescence in host cells and does not require post-translational modification. It is fast, reliable and convenient to detect. It is an ideal choice for the detection of firefly luciferase reporter gene system. Bio- One ^TM The One-Step Firefly Luciferase Assay Kit is a fast, stable, and consistent one-step detection kit developed for firefly luciferase . It has high detection sensitivity and is very suitable for high-throughput and ultra-high-throughput (96/384/1536 well) detection of firefly luciferase reporter gene expression in mammalian cells. This kit uses D-luciferin as a substrate. In the presence of ATP, magnesium ions, and oxygen, it can be catalyzed by firefly luciferase to undergo oxidative decarboxylation to produce activated oxyluciferin and bioluminescence (Figure 1). Bioluminescence is proportional to the promoter activity of luciferase and indirectly reflects the biological activity of the cell stimulant.

Figure 1. Luminescence mechanism of firefly luciferase substrate

luminescence value attenuation of different luciferase detection kits is shown in Figure 2.

Note: The actual readings may vary due to differences in cell number, transfection effect, detection instrument, etc. The data in the figure are for reference only.