Overview:

IgMCOUPER is an IgM-specific enzyme that digests human IgM at a specific site (..VPDQDT / AIRVFA..) below the CH2 domain in the heavy chain to produce F(ab')2 and Fc fragments. Enzymatic cleavage is complete within 30 minutes, and due to the specificity of the enzyme, there is no risk of over-digestion even with extended incubation times. The enzyme is active at pH 5.5 to 9.0 and does not require reducing conditions or cofactors for activity. Optimal activity is at 37°C, and increased incubation time is required if used at room temperature.
IgMCOUPER is recombinantly expressed in E. coli with a His tag and a molecular weight of 38kDa. The enzyme is capable of enzymatic cleavage of complex and high molecular weight human IgM, thereby facilitating IgM characterization during the development of vaccines, therapeutic drugs and diagnostic tools.
Enzyme activity definition :

IgMCOUPER digests ≥90% of 1 μg of human myeloma IgM when incubated in PBS (10 mM sodium phosphate, 140 mM NaCl, 2.7 mM KCl ), pH 7.4, at room temperature for 20 minutes.

Reagent packaging:

IgMCOUPER packaging specifications are as follows:

Note: Enzyme is provided as a lyophilized powder and is reconstituted by adding water.

Assay buffer: 10 mM sodium phosphate, 140 mM NaCl, 2.7 mM KCl , pH 7.4.

Product Features:

1. High specificity: only recognizes a specific digestion site in the human IgM heavy chain;

2. High specific activity: can quickly generate uniform F(ab')2 and Fc fragments within 30 minutes ;

3. High stability: Each batch of products undergoes strict quality control to achieve product batch-to-batch stability . Achieve intermediate characterization and quality control of IgM.

Storage conditions:

Please store the product at -20 °C upon receipt; after reconstitution, store at 4-8 °C for 1 month. Avoid repeated freezing and thawing.