background:

IgdE protease is an immunoglobulin degrading enzyme that cleaves only at a specific site above the hinge region of human IgG1 to produce Fab and intact Fc fragments. The optimal pH of the enzyme is 6.5~7.5, and no reducing environment or other auxiliary factors are required. Since IgdE protease only cleaves at a specific site (..KSCDKT / HTCPPCP..) of human IgG1, there is no risk of over-digestion by extending the incubation time.

Specifications:

Rhinogen ^® IgdE protease packaging specifications are as follows:

QIP-007 is a lyophilized powder formulation product with a formulation buffer of 10mM PB, 137mM NaCl, 2.7mM KCl , pH 7.4, and does not contain preservatives.

Product Source:

Rhinogen ^® IgdE protease is recombinantly expressed using the E. coli expression system and has a molecular weight of approximately 70 kDa .

Product quality:

SDS-PAGE analysis showed that the purity was ≥95%; no contaminating exoglycosidase, endoglycosidase and protease activities were detected.