background:

Lys-C (Lysyl endopeptidase) belongs to the serine protease family and can specifically hydrolyze the peptide bond at the carboxyl end of lysine residues. Lys-C has the best activity in the pH range of 7.0-9.0. The hydrolysis reaction can be carried out in solution or in gel.

Rhinogen ^® Endoproteinase Lys-C is a recombinant lysyl endonuclease expressed by E. coli, and does not contain other proteases (such as serine proteases such as Lys-N and Arg-C). It is specific for lysyl peptide bond cleavage, including fully specific cleavage containing DK and PK sequences.

Packing Specifications:

Rhinogen ^® Endoproteinase Lys-C packaging specifications are as follows:

QIP-004 is provided as a lyophilized powder.

Product Source:

Rhinogen ^® Endoproteinase Lys-C is a recombinant lysyl endonuclease expressed using the E. coli expression system, with a molecular weight of 31±3.1kD.

Product Quality:

Purity: single main band by SDS-PAGE; HPLC ≥ 90%.

Product Features:

The optimum pH is 7.0~9.5.

Enzyme activity definition :

required to catalyze the production of 1 μmol AC-Lys- pNA within 1 minute at 37°C and pH 9.0 is one activity unit.

Storage conditions:

1. The product is transported with ice packs or dry ice. Please store it below -20℃ immediately after receiving the product. The validity period is two years.

2. After dissolving in deionized water or 25mmol/L Tris-HCl (pH8.5), it can be stored at 2~8℃ for 7 days.

3. It is recommended to dilute with 25mmol/L Tris-HCl (pH8.5) to the required concentration and then package it. Store it at -20℃ and use it within 6 months. Avoid repeated freezing and thawing after dissolution.

Features:

Rhinogen ^® Endoproteinase Lys-C is a very stable recombinant protease suitable for protein sequence analysis in proteomics research.

1. No animal origin: Recombinant production, no exogenous virus contamination, and no animal origin raw materials are used in the production process

2. Freeze-dried powder: easy to store and transport;

3. Enhance digestion: Used with trypsin to reduce incomplete protein digestion;

4. High stability: Each batch of products undergoes strict quality control to achieve batch-to-batch stability .

Application:

1. Protein digestion and protein sequencing;

2. Enzyme-catalyzed synthesis of Lys-X compounds.

Recommended use:

1. Before use, please put Rhinogen ^® Take out Endoproteinase Lys-C and centrifuge at 10,000 rpm for 10 seconds to ensure that all the dry powder is at the bottom of the tube.

2. Dissolve the lyophilized powder in deionized water or 25mmol/L Tris-HCl (pH8.5) to obtain a Lys-C solution with a concentration of 0.1~1mg/mL.

Instructions:

This product is for research use only and is not intended for human or animal diagnostic or therapeutic use.