background:

Antibody glycosylation remodeling is a technology that changes the glycosylation pattern on antibody molecules. Its advantages include enhancing the stability of antibodies, improving their pharmacokinetic properties, and enhancing their antibody-dependent cellular cytotoxicity (ADCC) activity. This technology has a wide range of applications in drug development and treatment, including the treatment of cancer, autoimmune diseases, and infectious diseases.

Human IgG sugar chain remodeling and glycosylation refer to the change of structure or properties of human immunoglobulin G (IgG) molecules, or the introduction of chemical groups or functional groups at specific positions to achieve specific functions or applications. The significance of this remodeling or coupling is to expand the application field and function of human IgG and improve its therapeutic effect or biological activity. By changing the structure or properties of IgG, drug delivery performance, immune activity, stability, etc. can be regulated to achieve better therapeutic effects or application effects.

Product Packaging:

The Rhinogen ^® TransCOUPER ™ Azide Activation Kit packaging specifications are as follows:

Product Source:

The enzymes in the kit are all expressed in E. coli and have a His tag.

Product Features:

1. Facilitates custom site-specific conjugation of human IgG, with a degree of labeling (DOL) of 4;

2. Higher DOL improves sensitivity, allowing for better detection of low-altitude targets;

3. Fc-specific labeling can retain antigen binding ability;

4. Azide-activated human IgG can be generated within a few hours.

Enzyme activity definition :

The transglycosylation rate of 100 µg human IgG1 using the Rhinogen ^® TransCOUPER ™ Azide Activation Kit was ≥ 95% as determined by LC-MS .

Storage conditions:

Refrigerated transportation: After the product arrives, lmmobilizedEndoS2, Microspin and ProteinA purification columns should be stored at 2~8℃, and Glycosyltransferase and Glycoform Azoture hIgG1/4 or hIgG2 should be stored at -25~-5℃. Do not freeze lmmobilizedEndoS2, Microspin and ProteinA purification columns.

application:

Can be used for site-specific azide activation of human IgG.

Enzyme digestion principle:

Figure 1. Schematic diagram of the Rhinogen ^® TransCOUPER ™ Azide Activation Kit.

Precautions for use:

the lmmobilized EndoS2, Microspin and Protein A purification columns to room temperature before use ;

2. Before centrifugation, remove the bottom cover and slightly open the top cover;

3. This product is for research and development use only.