background:

Antibody glycosylation remodeling is a technology that changes the glycosylation pattern on antibody molecules. Its advantages include enhancing the stability of antibodies, improving their pharmacokinetic properties, and enhancing their antibody-dependent cellular cytotoxicity (ADCC) activity. This technology has a wide range of applications in drug development and treatment, including the treatment of cancer, autoimmune diseases, and infectious diseases.

Human IgG sugar chain remodeling and glycosylation refer to the change of structure or properties of human immunoglobulin G (IgG) molecules, or the introduction of chemical groups or functional groups at specific positions to achieve specific functions or applications. The significance of this remodeling or coupling is to expand the application field and function of human IgG and improve its therapeutic effect or biological activity. By changing the structure or properties of IgG, drug delivery performance, immune activity, stability, etc. can be regulated to achieve better therapeutic effects or application effects.

The Rhinogen ^® TransCOUPER ™ Glycan Remodeling Kit can achieve glycan remodeling of specific sites of natural human IgG within a few hours , and transglycosylate the N-sugar at the Fc end of the antibody through enzymatic remodeling technology, thereby forming a homologous antibody pool carrying a specific glycoform . The kit can be used to generate antibodies carrying G0, G1, G2 or G2S2 glycoforms .

The Rhinogen ^® TransCOUPER ™ Glycan Remodeling Kit contains enough material to transglycosylate 1 mg of human IgG. The remodeled sample has the same degree of fucosylation as the original molecule. If removal of core fucosylation is required, we recommend the Rhinogen ^® TransCOUPER ™ Defucosylation Remodeling Kit.

Product Packaging:

the Rhinogen ^® TransCOUPER ™ Glycan Remodeling Kit are as follows:

Product Source:

The enzymes in the kit are all expressed in E. coli and have a His tag.

Product Features:

Rapid and efficient generation of human IgG with homogeneous glycoforms ;

Facilitates functional analysis of specific glycan structures;

reliable glycan remodeling within hours .

principle:

Note:

1. The reagent can be used for all subclasses of human IgG. However, the reaction on IgG2 is slightly slower, and a longer incubation time may be required to obtain more than 95% transglycosylation;

2. Equilibrate the lmmobilized EndoS2, Microspin and Protein A purification columns to room temperature before use;

3. Before centrifugation, remove the bottom cover and slightly open the top cover;

4. This product is for research and development use only.