Product Background :

Endoglycosidase F1 (Endo F1) cleaves between two N-acetylglucosamine residues in the core of oligosaccharide N-linked diacetylchitobiose glycans, generating truncated sugar molecules with one N-acetylglucosamine residue remaining on asparagine. Endo F1 cleaves asparagine-linked or free high-mannose ( oligomannose ) and hybrid structures, but not complex oligosaccharides. Core fucosylation of hybrid structures reduces the cleavage rate of Endo F1 by more than 50-fold. In addition, Endo F1 hydrolyzes sulfates containing high-mannose chains and can be used under native or non-denaturing deglycosylation conditions . The enzyme is suitable for deglycosylation from glycoproteins under native conditions and is suitable for a variety of glycomics , proteomics, and mass spectrometry applications.

Rhinogen ^® Endo F1 is recombinantly expressed in E. coli with a 6×His tag at the C-terminus . Unlike Endo H, Endo F1 can cleave high mannose and some hybrid N-glycans from peptides and proteins.

Specifications :

Rhinogen ^® Endoglycosidase F1 (Endo F1) packaging specifications are as follows:

Supporting reagents:

Rhinogen ^® Endoglycosidase F1 (Endo F1) are as follows:

Product Source :

Endo F1 is recombinantly expressed in E. coli , with a theoretical molecular weight of approximately 32 kDa and a 6×His tag at the C- terminus .

Product quality :

1. High purity: detected by SDS-PAGE, purity ≥90%;

2. High activity: activity ≥16.5U/ml

3. High stability: Each batch of products undergoes strict quality control to achieve product batch-to-batch stability .

Enzyme activity definition :

The amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μM denatured ribonuclease B (RNase B) at 37˚C, pH 5.5 in 1 minute .

application:

1. Glycomics ;

2. Glycoform analysis and determination of glycosylation sites;

3. Proteomics;

4. Mass spectrometry applications.

Storage conditions:

Transported in ice packs, can be stored at 2-8℃ for 12 months. Avoid repeated freezing and thawing.

Precautions for use:

1. For different glycoprotein samples, it is necessary to experiment to find the most suitable enzyme concentration and reaction time.

2. The reaction system can be expanded linearly.

3. To prevent microbial contamination, use sterile methods whenever possible.

4. Higher enzyme concentrations can improve the digestion efficiency of individual glycoproteins and need to be optimized based on actual conditions.

5. Appropriate subpackaging can be performed to reduce the loss of activity caused by multiple freeze-thaw cycles.

6. This product is for research use only and is not intended for human or animal diagnosis or treatment.

Frequently asked questions

Q1: What is a good positive control for Endo F1?

A1: RNase B can be used as a positive control for Endo F1, and the cleavage effect can be visually observed by SDS-PAGE.

Q2: What is the difference between PNGase F and Endo F3?

A2: Endoglycosidase F1 hydrolyzes sulfate containing high mannose chains by cleaving between two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, producing a truncated sugar molecule with one N-acetylglucosamine residue remaining on asparagine. In contrast, PNGase F removes the oligosaccharide intact.