background:

Fucose exists in different linkages on N- and O-glycans and is an important component of many oligosaccharide (O- or N-linked) and polysaccharide structures, as well as glycoproteins and glycolipids, which are often involved in various biological events, including fertilization, embryogenesis, apoptosis, signal transduction, and disease progression, such as rheumatoid arthritis, inflammation, cancer, and cystic fibrosis.

Rhinogen ^® α1-3,4 fucosidase is recombinantly expressed in E. coli. It has an optimum pH of 6.0 and a working pH range between 5.5 and 7, within which the enzyme maintains at least 50% of its maximal activity. The enzyme efficiently recognizes α1-3,4 fucosylated glycans (e.g. Lewis X/A epitopes, including their sialylated counterparts) and hydrolyzes terminal α1-3 and α1-4 fucosyl bonds in these substrates without removing the sialic acid moiety. Since no sialidase is required prior to targeted digestion of antennary fucosylation, this enzyme can be used to simplify the workflow, making it both economical and time-saving. Another advantage is that the enzyme can be used to monitor α1-3,4 fucosylation on both free glycans and glycoproteins, eliminating the need for chemical or enzymatic release of glycans from glycoproteins.

Reagent packaging:

Rhinogen ^® α1-3,4 Fucosidase (α1-3,4 Fucosidase ) packaging specifications are as follows:

The product is stored in 200 mM citrate buffer pH 6.0 containing 250 mM NaCl, without added preservatives.

Each vial can digest up to 750μg glycoprotein or 250μg free glycans.

Supporting reagents:

The reagents provided with Rhinogen ® ^α1-3,4 Fucosidase are as follows:

Product Source:

Recombinantly expressed in E. coli , with a theoretical molecular weight of approximately 62kDa and a 6×His tag at the C-terminus .

Product quality:

SDS-PAGE analysis showed that the purity was ≥95%; no contaminating exoglycosidase, endoglycosidase and protease activities were detected.

Enzyme activity definition :

hydrolyzes ≥90% of fucose from 4.5 nmol 3'-fucosyllactose when incubated in 50 mM sodium acetate, pH 6.0 at 37°C for 30 min.

Storage conditions:

1. Use ice packs for transportation. Please place the enzyme at 2-8℃ immediately after receiving the product; the supporting reagents should be stored at -20℃.

2. This product does not contain preservatives. Please ensure aseptic operation to avoid contamination.

characteristic:

Rhinogen ^® α1-3,4- fucosidase is a highly purified and very stable exoglycosidase that can act on oligosaccharides, complex glycans and glycoprotein samples, such as those displaying (sialylated) Lewis X/A epitopes. Both fluorescently labeled and unlabeled glycans are suitable. It has high stability and is compatible with downstream assays.

1. High purity: no contaminating protease/other glycosidase, purity ≥95%;

2. High stability: Each batch of products undergoes strict quality control to achieve product batch-to-batch stability ;

3. Compatible with downstream HPLC and mass spectrometry: It does not contain glycerol, and the enzyme digestion system is compatible with the HPLC and mass spectrometry workflow environment.

application:

1. Glycan structure analysis;

2. Characterization and quality control of therapeutic recombinant proteins;

3. Eliminate glycoprotein heterogeneity.

Note:

This product is for research use only and is not intended for diagnostic or therapeutic use in humans or animals.