background:

Fucose is an important component of many oligosaccharide (O- or N-linked) and polysaccharide structures, as well as glycoproteins and glycolipids, which are often involved in various biological events, including fertilization, embryogenesis, apoptosis, signal transduction, and disease progression, such as rheumatoid arthritis, inflammation, cancer, and cystic fibrosis. Defucosylation of glycoconjugates is an important process to understand the biological effects of glycoconjugates .

Rhinogen ^® α1-2,4,6 Fucosidase is a recombinant glycosidase expressed in E. coli and purified by multi-step chromatography. Its molecular weight is about 50kDa. To facilitate the combination with other glycosidases, the enzyme activity of Rhinogen ^® α1-2,4,6 Fucosidase is calculated at 37°C. However, the optimal reaction temperature of the enzyme is 50°C. At 37°C, the activity will be reduced by about 35%.

Packing Specifications:

Rhinogen ^® α1-2,4,6 Fucosidase (α1-2,4,6 Fucosidase ) packaging specifications are as follows:

The product is stored in 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5, without added preservatives.

Supporting reagents:

The reagents provided with Rhinogen ^® α1-2,4,6 Fucosidase are as follows:

Product Source:

The sequence is derived from Omnitrophica bacterium and recombinantly expressed in E. coli . The theoretical molecular weight is about 50KD, and the C- terminus has a 6×His tag.

Product quality:

SDS-PAGE analysis showed that the purity was ≥95%; no contaminating exoglycosidase, endoglycosidase and protease activities were detected.

Enzyme activity definition :

One unit of enzyme activity is defined as the amount of enzyme required to cleave > 95% of fucose from 1 nmol G0F of human IgG [ GlcNAc β1-2Manα1-6( GlcNAc β1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc( Fuc α1-6)-AMAC] in a total reaction volume of 10 µl for 1 hour at 37°C.

Storage conditions:

1. Use ice packs for transportation. Please place the enzyme at 2-8℃ immediately after receiving the product; the supporting reagents should be stored at -20℃.

2. This product does not contain preservatives. Please ensure aseptic operation to avoid contamination.

Product Features:

Rhinogen ^® α1-2,4,6 Fucosidase is a broadly specific exoglycosidase that catalyzes the hydrolysis of α1-2, α1-4, and α1-6 terminal fucose residues in oligosaccharides and cleaves α1-6 fucose residues more efficiently than other bonds.

1. High purity: no contaminating protease/other glycosidase, purity ≥95%;

2. Stability: Each batch of products undergoes strict quality control and exhibits high stability;

3. Compatible with downstream HPLC and mass spectrometry: It does not contain glycerol, and the enzyme digestion system is compatible with the HPLC and mass spectrometry workflow environment.

application:

1. Glycan structure analysis;

2. Characterization and quality control of therapeutic recombinant proteins;

3. Eliminate glycoprotein heterogeneity.